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Addgene

pFSBC30
(Plasmid #44794)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44794 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FREQ-Seq Barcode: GCTTAG
  • Species
    Synthetic

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

These plasmids are used to generate bridging primers with Illumina-compatible barcodes for allele frequency sequencing (FREQ-Seq). Similar to RNA-Seq, the FREQ-Seq method can rapidly determine allele frequencies directly from mixed populations with minimal library preparation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFSBC30 was a gift from Christopher Marx (Addgene plasmid # 44794 ; http://n2t.net/addgene:44794 ; RRID:Addgene_44794)

  • For your References section:

    FREQ-Seq: a rapid, cost-effective, sequencing-based method to determine allele frequencies directly from mixed populations. Chubiz LM, Lee MC, Delaney NF, Marx CJ. PLoS One. 2012;7(10):e47959. doi: 10.1371/journal.pone.0047959. Epub 2012 Oct 31. 10.1371/journal.pone.0047959 PubMed 23118913
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