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Addgene

pBS-85xtetO-KlURA3
(Plasmid #44757)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44757 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBlueScriptII (SK+)
  • Backbone size w/o insert (bp) 2900
  • Total vector size (bp) 8048
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    85x tetO array + KlURA3 marker + CHRI1 homology
  • Insert Size (bp)
    5167

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ApaI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer M13 forward
  • 3′ sequencing primer M13 reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-85xtetO-KlURA3 was a gift from Narendra Maheshri (Addgene plasmid # 44757 ; http://n2t.net/addgene:44757 ; RRID:Addgene_44757)

  • For your References section:

    Harnessing mutagenic homologous recombination for targeted mutagenesis in vivo by TaGTEAM. Finney-Manchester SP, Maheshri N. Nucleic Acids Res. 2013 Mar 7. 10.1093/nar/gkt150 PubMed 23470991
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