Skip to main content
Addgene

PRS415-Gal1pr-sctetR-FokI
(Plasmid #44756)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44756 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    PRS415
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 8442
  • Modifications to backbone
    removed NgoMIV-KpnI fragment by blunt end ligation
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sctetR-FokI
  • Species
    Synthetic
  • Insert Size (bp)
    1965
  • Entrez Gene
    fokI (a.k.a. HCBAA847_2198)
  • Promoter Gal1pr

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer CTCTATACTTTAACGTCAAGGAG
  • 3′ sequencing primer GAATGTAAGCGTGACATAAC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PRS415-Gal1pr-sctetR-FokI was a gift from Narendra Maheshri (Addgene plasmid # 44756 ; http://n2t.net/addgene:44756 ; RRID:Addgene_44756)

  • For your References section:

    Harnessing mutagenic homologous recombination for targeted mutagenesis in vivo by TaGTEAM. Finney-Manchester SP, Maheshri N. Nucleic Acids Res. 2013 Mar 7. 10.1093/nar/gkt150 PubMed 23470991
Related items:
Commonly requested with: