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Addgene

Flag-p50(1-435)
(Plasmid #44747)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44747 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEYFP-C1
  • Backbone manufacturer
    Clontech
  • Modifications to backbone
    YFP replaced with FLAG.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    p50
  • Species
    M. musculus (mouse)
  • Mutation
    AA 1-435
  • Entrez Gene
    Nfkb1 (a.k.a. NF-KB1, NF-kappaB, NF-kappaB1, p105, p50, p50/p105)
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer CMV Fwd
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene's sequencing results identified a K352N mutation in the p50 insert sequence compared to GenBank reference sequence NP_032715.2.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag-p50(1-435) was a gift from Gourisankar Ghosh (Addgene plasmid # 44747 ; http://n2t.net/addgene:44747 ; RRID:Addgene_44747)

  • For your References section:

    The Nfkb1 and Nfkb2 proteins p105 and p100 function as the core of high-molecular-weight heterogeneous complexes. Savinova OV, Hoffmann A, Ghosh G. Mol Cell. 2009 Jun 12;34(5):591-602. doi: 10.1016/j.molcel.2009.04.033. 10.1016/j.molcel.2009.04.033 PubMed 19524538
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