Skip to main content
Addgene

pCI-MEG3-del5
(Plasmid #44732)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44732 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCI
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4006
  • Total vector size (bp) 5159
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Maternally Expressed Gene 3
  • Alt name
    MEG3-del5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1200
  • Mutation
    The first 450 nt were deleted from the MEG3 cDNA.
  • Entrez Gene
    MEG3 (a.k.a. FP504, GTL2, LINC00023, NCRNA00023, PRO0518, PRO2160, onco-lncRNA-83, prebp1)
  • Promoter hCMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (destroyed during cloning)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pCI-5F
  • 3′ sequencing primer pCI-3R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-MEG3-del5 was a gift from Anne Klibanski (Addgene plasmid # 44732 ; http://n2t.net/addgene:44732 ; RRID:Addgene_44732)

  • For your References section:

    Activation of p53 by MEG3 non-coding RNA. Zhou Y, Zhong Y, Wang Y, Zhang X, Batista DL, Gejman R, Ansell PJ, Zhao J, Weng C, Klibanski A. J Biol Chem. 2007 Aug 24;282(34):24731-42. Epub 2007 Jun 13. 10.1074/jbc.M702029200 PubMed 17569660
Related items:
Commonly requested with: