pcDNA3.1-SC35-cMyc
(Plasmid #44721)

• Depositing Lab: Kathleen Scotto
• Publication: Shi et al Mol Cell Biol. 2008 Jan;28(2):883-95. Epub 2007 Nov 19.

Backbone

• Vector backbone: pcDNA3.1/myc-His (-)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 6160
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): JM109
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SC35
• Alt name: SFRS2
• Species: H. sapiens (human)
• Insert Size (bp): 666
• GenBank ID: NM_003016.4 NP_003007.2
• Entrez Gene: SRSF2 (a.k.a. PR264, SC-35, SC35, SFRS2, SFRS2A, SRp30b)
• Promoter: CMV
• Tags / Fusion Proteins:
  • myc (C terminal on backbone)
  • his (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: Bam HI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Both tags are in frame with the insert.