pJDS74-GG-NNN
(Plasmid #44715)

• Purpose: (Empty Backbone)
• Depositing Lab: Nathan Lawson
• Publication: Gupta et al Genome Res. 2013 Mar 11.

Backbone

• Vector backbone: pJDS74
• Backbone size (bp): 6433
• Modifications to backbone: 1)To make pJDS vectors compatible with the Voytas lab Golden Gate kit, the overhangs generated by Esp3I cleavage sites in the pJDS-GG vectors were adjusted to GGGA/CTAT to ensure proper complementarity between intermediate vectors and the final vector. 2)Each pJDS-GG vector is modified to match the reading frame of the Golden Gate assembled intermediate arrays.
• Vector type: Mammalian Expression
• Promoter: CMV
• Tags / Fusion Proteins:
  • 3X Flag (N terminal on backbone)
  • FOKI WT (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Cloning Information

• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Supplemental Documents:
  • Modified Golden Gate Protocol for pJDS vectors
• A portion of this plasmid was derived from a plasmid made by: Plasmid derived from pJDS74 from Addgene "Joung Lab TAL Effector Engineering Reagents" Kit.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is modified pJDS74 vector from "Joung Lab TAL Effector Engineering Reagents" Kit. Modifications are made to the backbone to make it compatible with Voytas Golden Gate TALEN Kit and it can be used in place of pTAL3 or pTAL4. Please see the attached protocol below for modifications.