PL08009A1B07
(Plasmid
#44673)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 44673 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript SK II (+)
- Backbone size w/o insert (bp) 2961
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSmed-pp2
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Alt nameEST Contig 6203
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Alt namePL06021B2G05
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SpeciesSchmidtea mediterranea
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GenBank IDHO006984.1 DN315773.1
- Promoter T7 sense, T3 antisense
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PL08009A1B07 was a gift from Phillip Newmark (Addgene plasmid # 44673 ; http://n2t.net/addgene:44673 ; RRID:Addgene_44673) -
For your References section:
Molecular markers to characterize the hermaphroditic reproductive system of the planarian Schmidtea mediterranea. Chong T, Stary JM, Wang Y, Newmark PA. BMC Dev Biol. 2011 Nov 10;11:69. 10.1186/1471-213X-11-69 PubMed 22074376