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Addgene

PL08004B2A05
(Plasmid #44665)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44665 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript SK II (+)
  • Backbone size w/o insert (bp) 2961

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Smed-msy4
  • Alt name
    EST Contig 5455
  • Alt name
    PL06012A1D09
  • Species
    Schmidtea mediterranea
  • GenBank ID
    HO007850.1 DN312133.1
  • Promoter T7 sense, T3 antisense

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PL08004B2A05 was a gift from Phillip Newmark (Addgene plasmid # 44665 ; http://n2t.net/addgene:44665 ; RRID:Addgene_44665)

  • For your References section:

    Molecular markers to characterize the hermaphroditic reproductive system of the planarian Schmidtea mediterranea. Chong T, Stary JM, Wang Y, Newmark PA. BMC Dev Biol. 2011 Nov 10;11:69. 10.1186/1471-213X-11-69 PubMed 22074376
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