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Addgene

pJMD-In3.4:EGFP
(Plasmid #44662)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44662 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDestTol2pA2
  • Backbone size w/o insert (bp) 5883
  • Total vector size (bp) 6200
  • Vector type
    Tol2 vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Angptl4 Intronic Region 3.4
  • Alt name
    In3.4
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    314
  • Promoter cFOS

Cloning Information for Gene/Insert 1

  • Cloning method Gateway Cloning
  • 5′ sequencing primer In3.4 F primer
  • 3′ sequencing primer EGFP R primer
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Minimal cFOS
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    100

Gene/Insert 3

  • Gene/Insert name
    EGFP
  • Insert Size (bp)
    1000

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJMD-In3.4:EGFP was a gift from John Rawls (Addgene plasmid # 44662 ; http://n2t.net/addgene:44662 ; RRID:Addgene_44662)
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