pcDNA3-MTS-CA-c-Src-FLAG
(Plasmid #44654)

• Depositing Lab: Yoshimi Homma
• Publication: Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 7260
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC)
• Species: H. sapiens (human)
• Insert Size (bp): 1608
• Mutation: changed Tyr 530 to Phe
• GenBank ID: NM_005417
• Entrez Gene: SRC (a.k.a. ASV, SRC1, THC6, c-SRC, p60-Src)
• Promoter: CMV promoter
• Tags / Fusion Proteins:
  • mitochondria-targeting sequence (MTS: residues 1-40 of the amino acid sequence of very long chain acyl-CoA dehydrogenase) (N terminal on insert)
  • FLAG tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bgl II (not destroyed)
• 3′ cloning site: Xho I (not destroyed)
• 5′ sequencing primer: aaagccacttgcctaggga
• 3′ sequencing primer: cgcctgtgcctagaggtt

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry