pFA6a-link-PhiYellow-SpHis5
(Plasmid
#44651)
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PurposeYeast tagging vector to create gene fusion with PhiYellow with SpHis5 selection
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 44651 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFA6a-link
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Vector typeYeast Expression
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namephiYellow
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Insert Size (bp)702
Resource Information
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A portion of this plasmid was derived from a plasmid made byphiYellow was purchased from Evrogen.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This vector is derived from the sequences published in “Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae.” Sheff MA, Thorn KS. Yeast. 2004 Jun;21(8):661-70.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFA6a-link-PhiYellow-SpHis5 was a gift from Kurt Thorn (Addgene plasmid # 44651 ; http://n2t.net/addgene:44651 ; RRID:Addgene_44651)