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Addgene

pFA6a-link-yETSapphire-CaUra3
(Plasmid #44650)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44650 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFA6a-link
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Turbo Sapphire
  • Alt name
    T-Sapphire

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This vector is derived from the sequences published in “Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae.” Sheff MA, Thorn KS. Yeast. 2004 Jun;21(8):661-70.

yETSapphire was generated by mutagenizing yEGFP to the sequence published in "Efficiently folding and circularly permuted variants of the Sapphire mutant of GFP." Zapata-Hommer O, Griesbeck O. BMC Biotechnol. 2003 May 22;3:5. Epub 2003 May 22.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFA6a-link-yETSapphire-CaUra3 was a gift from Kurt Thorn (Addgene plasmid # 44650 ; http://n2t.net/addgene:44650 ; RRID:Addgene_44650)
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