pFA6a-link-yEPAGFP-CaUra3
(Plasmid
#44649)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 44649 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFA6a-link
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameyEPAGFP
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Alt nameyeast codon optimized photoactivatible GFP
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Insert Size (bp)717
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This vector is derived from the sequences published in “Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae.” Sheff MA, Thorn KS. Yeast. 2004 Jun;21(8):661-70.
PAGFP was made my mutagenizing yEGFP to the sequence published in "A photoactivatable GFP for selective photolabeling of proteins and cells." Patterson GH, Lippincott-Schwartz J. Science. 2002 Sep 13;297(5588):1873-7.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFA6a-link-yEPAGFP-CaUra3 was a gift from Kurt Thorn (Addgene plasmid # 44649 ; http://n2t.net/addgene:44649 ; RRID:Addgene_44649)
