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Addgene

pFA6a-link-yECerulean-CaUra3
(Plasmid #44648)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44648 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFA6a-link
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    yECerulean
  • Alt name
    yeast codon optimized Cerulean variant of CFP
  • Insert Size (bp)
    717

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This vector is derived from the sequences published in “Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae.” Sheff MA, Thorn KS. Yeast. 2004 Jun;21(8):661-70.

yECerulean was generated by mutagenizing yEGFP to the sequence published in "An improved cyan fluorescent protein variant useful for FRET." Rizzo MA, Springer GH, Granada B, Piston DW. Nat Biotechnol. 2004 Apr;22(4):445-9. Epub 2004 Feb 29.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFA6a-link-yECerulean-CaUra3 was a gift from Kurt Thorn (Addgene plasmid # 44648 ; http://n2t.net/addgene:44648 ; RRID:Addgene_44648)
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