pFA6a-link-mCherry-SpHis5
(Plasmid #44643)

• Purpose: Yeast tagging vector to create gene fusion with mCherry with SpHis5 selection
• Depositing Lab: Kurt Thorn
• Publication: Yeast tagging plasmids. (unpublished)

Backbone

• Vector backbone: pFA6a-link
• Vector type: Yeast Expression
• Selectable markers: HIS3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mCherry
• Alt name: monomeric Cherry
• Insert Size (bp): 711

Resource Information

• A portion of this plasmid was derived from a plasmid made by: mCherry is covered by an MTA from Roger Tsien's lab: http://www.tsienlab.ucsd.edu/Samples.htm

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This vector is derived from the sequences published in “Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae.” Sheff MA, Thorn KS. Yeast. 2004 Jun;21(8):661-70.