pFA6a-link-mKO1-CaUra3
(Plasmid #44638)

• Purpose: Yeast tagging vector to create gene fusion with mKO1 with CaUra3 selection
• Depositing Lab: Kurt Thorn
• Publication: Yeast tagging plasmids. (unpublished)

Backbone

• Vector backbone: pFA6a-link
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mKO1
• Alt name: monomeric Kusabira orange 1
• Insert Size (bp): 657

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (not destroyed)
• 3′ cloning site: AscI (not destroyed)
• 5′ sequencing primer: N/A

Resource Information

• A portion of this plasmid was derived from a plasmid made by: mKO1 was purchased from MBL International.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This vector is derived from the sequences published in “Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae.” Sheff MA, Thorn KS. Yeast. 2004 Jun;21(8):661-70.