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Addgene

PU.1-W215G LZRS
(Plasmid #44635)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44635 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    LZRS
  • Backbone size w/o insert (bp) 12443
  • Total vector size (bp) 13300
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    very slow growing--allow extra time to propagate.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PU.1 W215G
  • Alt name
    SFFV proviral integration 1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    839
  • Mutation
    215 mutation (W to G)
  • Entrez Gene
    Spi1 (a.k.a. Dis-1, Dis1, PU.1, Sfpi-1, Sfpi1, Spi-1, Tcfpu1, Tfpu.1)
  • Tags / Fusion Proteins
    • IRES (C terminal on backbone)
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer LZRS-F
  • 3′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mouse PU.1 coding sequence with 215 mutation (W to G) that abolishes DNA binding. Subcloned from LZRS PU.1-WT (Addgene plasmid #34835).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PU.1-W215G LZRS was a gift from Ellen Rothenberg (Addgene plasmid # 44635 ; http://n2t.net/addgene:44635 ; RRID:Addgene_44635)

  • For your References section:

    Constitutive expression of PU.1 in fetal hematopoietic progenitors blocks T cell development at the pro-T cell stage. Anderson MK, Weiss AH, Hernandez-Hoyos G, Dionne CJ, Rothenberg EV. Immunity. 2002 Feb;16(2):285-96. 10.1016/S1074-7613(02)00277-7 PubMed 11869688
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