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PU.1-S148A LZRS
(Plasmid #44633)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44633 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    LZRS
  • Backbone size w/o insert (bp) 12443
  • Total vector size (bp) 13300
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PU.1 S148A
  • Alt name
    SFFV proviral integration 1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    839
  • Mutation
    148 mutation (S to A)
  • Entrez Gene
    Spi1 (a.k.a. Dis-1, Dis1, PU.1, Sfpi-1, Sfpi1, Spi-1, Tcfpu1, Tfpu.1)
  • Tags / Fusion Proteins
    • IRES (C terminal on backbone)
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer LZRS-F
  • 3′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mouse PU.1 coding sequence with 148 mutation (S to A) that abolishes IRF family interactions. Subcloned from LZRS PU.1-WT (Addgene plasmid #34835).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PU.1-S148A LZRS was a gift from Ellen Rothenberg (Addgene plasmid # 44633 ; http://n2t.net/addgene:44633 ; RRID:Addgene_44633)

  • For your References section:

    Subversion of T lineage commitment by PU.1 in a clonal cell line system. Dionne CJ, Tse KY, Weiss AH, Franco CB, Wiest DL, Anderson MK, Rothenberg EV. Dev Biol. 2005 Apr 15;280(2):448-66. 10.1016/j.ydbio.2005.01.027 PubMed 15882585
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