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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 44630 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneNGFR (Addgene plasmid 27489)
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Backbone manufacturerWarren Pear lab
- Backbone size w/o insert (bp) 6500
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGfi1
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Alt namegrowth factor independent 1
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SpeciesM. musculus (mouse)
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Insert Size (bp)1284
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Entrez GeneGfi1 (a.k.a. Gfi-1, Pal-1, Pal1)
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Tags
/ Fusion Proteins
- IRES (C terminal on backbone)
- truncated NGFR (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer pBABE-5 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Gfi1 NGFR was a gift from Ellen Rothenberg (Addgene plasmid # 44630 ; http://n2t.net/addgene:44630 ; RRID:Addgene_44630) -
For your References section:
Architecture of a lymphomyeloid developmental switch controlled by PU.1, Notch and Gata3. Del Real MM, Rothenberg EV. Development. 2013 Mar;140(6):1207-19. doi: 10.1242/dev.088559. 10.1242/dev.088559 PubMed 23444353