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Addgene

PCIN4-PURO-K8
(Plasmid #44626)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44626 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCIN4-Puro
  • Modifications to backbone
    A PvuII to XbaI fragment of PGK puro was cloned into pCIN4 cut with SmaI and XbaI replacing Neo.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    K8
  • Alt name
    KRT8 keratin, type II cytoskeletal 8 isoform 2
  • Alt name
    3856
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_002273.3
  • Entrez Gene
    KRT8 (a.k.a. CARD2, CK-8, CK8, CYK8, K2C8, K8, KO)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer CMV Fwd
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene's sequencing results identified two silent mutations in K8 when compared to GenBank accession NM_002273.3. The protein sequence is not affected by these differences.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PCIN4-PURO-K8 was a gift from Robert Oshima (Addgene plasmid # 44626 ; http://n2t.net/addgene:44626 ; RRID:Addgene_44626)
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