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Addgene

MMTV-VEGF
(Plasmid #44625)

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44625 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    MMTV
  • Backbone manufacturer
    Philip Leder
  • Vector type
    Mammalian Expression, Mouse Targeting

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    VEGF121
  • Species
    M. musculus (mouse)
  • Mutation
    contains aa160-368; G165E mutation
  • GenBank ID
    NM_009505
  • Entrez Gene
    Vegfa (a.k.a. L-VEGF, Vegf, Vpf)
  • Promoter Mouse mammary tumor virus promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer NA
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MMTV-VEGF was a gift from Robert Oshima (Addgene plasmid # 44625 ; http://n2t.net/addgene:44625 ; RRID:Addgene_44625)
  • For your References section:

    Angiogenic acceleration of Neu induced mammary tumor progression and metastasis. Oshima RG, Lesperance J, Munoz V, Hebbard L, Ranscht B, Sharan N, Muller WJ, Hauser CA, Cardiff RD. Cancer Res. 2004 Jan 1;64(1):169-79. 10.1158/0008-5472.CAN-03-1944 PubMed 14729621