KFS2MT
(Plasmid #44624)

• Depositing Lab: Robert Oshima
• Publication: Cecena et al Am J Pathol. 2006 Jan;168(1):310-20.

Backbone

• Vector backbone: pGEM-1
• Vector type: Mouse Targeting

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: keratin 18 gene
• Species: H. sapiens (human)
• Entrez Gene: KRT18 (a.k.a. CK-18, CYK18, K18)
• Tag / Fusion Protein:
  • polyomavirus middle T antigen (PyMT)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NA (unknown if destroyed)
• 3′ cloning site: NA (unknown if destroyed)
• 5′ sequencing primer: Sp6
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Sanford-Burnham Medical Research Institute Ancillary Agreement
• Industry Terms:
  • Not Available to Industry

Depositor Comments

K18 gene with trimer poly A signals flanked on both sides by two loxp seq., followed by IRES seq and PyMT gene. Cre removes termination sequences permitting K18 gene to be transcribed and spliced normally with PyMT gene being expressed from second translational reading frame.

Human K18 gene from pGC1853 (Addgene #44619).