pK812
(Plasmid #44622)

• Depositing Lab: Robert Oshima
• Publication: Kulesh et al Mol Cell Biol. 1989 Apr;9(4):1553-65.

Backbone

• Vector backbone: pGEM-1
• Vector type: Cloning

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: K8
• Alt name: KRT8 keratin, type II cytoskeletal 8 isoform 2
• Alt name: Keratin 8
• Alt name: ENDO A
• Species: H. sapiens (human)
• Insert Size (bp): 1800
• GenBank ID: NM_002273.3
• Entrez Gene: KRT8 (a.k.a. CARD2, CK-8, CK8, CYK8, K2C8, K8, KO)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: Sp6
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Sanford-Burnham Medical Research Institute Ancillary Agreement
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results identified two silent mutations in K8 when compared to GenBank accession NM_002273.3. The protein sequence is not affected by these differences.