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Addgene

peceK8
(Plasmid #44621)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44621 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pECE
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    K8
  • Alt name
    KRT8
  • Alt name
    Keratin 8
  • Species
    H. sapiens (human)
  • Entrez Gene
    KRT8 (a.k.a. CARD2, CK-8, CK8, CYK8, K2C8, K8, KO)
  • Promoter SV40

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer SV40pro-F
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    peceK8 was a gift from Robert Oshima (Addgene plasmid # 44621 ; http://n2t.net/addgene:44621 ; RRID:Addgene_44621)
  • For your References section:

    Posttranslational regulation of keratins: degradation of mouse and human keratins 18 and 8. Kulesh DA, Cecena G, Darmon YM, Vasseur M, Oshima RG. Mol Cell Biol. 1989 Apr;9(4):1553-65. PubMed 2471065