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Addgene

LK444K18
(Plasmid #44620)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44620 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    LK444
  • Modifications to backbone
    For backbone details - see attached plasmid map
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    K18
  • Alt name
    KRT18
  • Species
    H. sapiens (human)
  • Entrez Gene
    KRT18 (a.k.a. CK-18, CYK18, K18)
  • Promoter human Beta-Actin

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer hBactin-F
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LK444K18 was a gift from Robert Oshima (Addgene plasmid # 44620 ; http://n2t.net/addgene:44620 ; RRID:Addgene_44620)

  • For your References section:

    Posttranslational regulation of keratins: degradation of mouse and human keratins 18 and 8. Kulesh DA, Cecena G, Darmon YM, Vasseur M, Oshima RG. Mol Cell Biol. 1989 Apr;9(4):1553-65. PubMed 2471065
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