pGC1853
(Plasmid
#44619)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 44619 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGC
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Vector typeCloning
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namekeratin 18 gene
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Alt namek18
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Alt nameKrt18
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SpeciesH. sapiens (human)
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Entrez GeneKRT18 (a.k.a. CK-18, CYK18, K18)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Whole human K18 gene including flanking regions and six exons. Best expression of K18 in transgenic or transfection
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGC1853 was a gift from Robert Oshima (Addgene plasmid # 44619 ; http://n2t.net/addgene:44619 ; RRID:Addgene_44619) -
For your References section:
Cloning of the human keratin 18 gene and its expression in nonepithelial mouse cells. Kulesh DA, Oshima RG. Mol Cell Biol. 1988 Apr;8(4):1540-50. PubMed 2454392
Map uploaded by the depositor.
