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Addgene

FNE2-A72
(Plasmid #44608)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44608 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FNpCDNA3
  • Backbone manufacturer
    Addgene #45346
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Ets2-A72
  • Species
    M. musculus (mouse)
  • Mutation
    THR-72 converted to Alanine
  • Entrez Gene
    Ets2 (a.k.a. Ets-2)
  • Promoter CMV
  • Tags / Fusion Proteins
    • FLAG (N terminal on backbone)
    • SV40 NLS (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRV (destroyed during cloning)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

FNE2 (Addgene #44603) with Thr 72 to Ala mutation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FNE2-A72 was a gift from Robert Oshima (Addgene plasmid # 44608 ; http://n2t.net/addgene:44608 ; RRID:Addgene_44608)

  • For your References section:

    Oncogenic Neu/ErbB-2 increases ets, AP-1, and NF-kappaB-dependent gene expression, and inhibiting ets activation blocks Neu-mediated cellular transformation. Galang CK, Garcia-Ramirez J, Solski PA, Westwick JK, Der CJ, Neznanov NN, Oshima RG, Hauser CA. J Biol Chem. 1996 Apr 5;271(14):7992-8. 10.1074/jbc.271.14.7992 PubMed 8626480
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