pcDNA3.1(-) MID51 4xMyc Hisx6
(Plasmid #44598)

• Depositing Lab: David Chan
• Publication: Loson et al Mol Biol Cell. 2013 Jan 2.

Backbone

• Vector backbone: pcDNA3.1(-)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 6975
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MiD51
• Alt name: mitochondrial dynamic protein of 51 kDa homolog
• Species: M. musculus (mouse)
• Entrez Gene: Mief1 (a.k.a. A230016E22, AI452372, Smcr7l)
• Tags / Fusion Proteins:
  • 4xMyc (C terminal on backbone)
  • 6xHis (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry