pFIN-EF1a-GFP:2A:CHER(HA)-WPRE
(Plasmid #44597)

• Depositing Lab: Susan Semple-Rowland
• Publication: Verrier et al PLoS One. 2011;6(5):e20553. doi: 10.1371/journal.pone.0020553. Epub 2011 May 27.

Backbone

• Vector backbone: pTY
• Backbone manufacturer: LJ Chang
• Backbone size w/o insert (bp): 8680
• Total vector size (bp): 11676
• Modifications to backbone: Final pFIN backbone: Added cPPT-FLAP sequence upstream of MCS in pTY to create pTYF backbone (Coleman et al 2002 Physiol Genomics 12:221-228). Added 2x250 chicken β-globin HS4 core sequence into 3'LTR of pTYF backbone to create pFIN (Semple-Rowland et al 2007 Mol Vis 13:2001-2011).
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EF1a-GFP:2A:CHER(HA)
• Alt name: human elongation factor-1 promoter
• Alt name: GFP:2A:CHER(HA) fusion protein
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 2996
• Promoter: human elongation factor -1a (pos 2528-3971)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

GFP-2A-mCherH was created using a three-step PCR strategy described by Symczak et al [46] that included the addition of a GSG linker sequence upstream of the PTV1 2A peptide sequence.

mCherry was tagged with HA to enable detection using HA antibody.