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Addgene

pcDNA3.1(-) MID49 4xMyc Hisx6
(Plasmid #44596)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44596 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(-)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 6948
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MiD49
  • Alt name
    mitochondrial dynamic protein of 49 kDa homolog
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Mief2 (a.k.a. AI482195, Gm11, Smcr7)
  • Tags / Fusion Proteins
    • 4xMyc (C terminal on backbone)
    • 6xHis (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1(-) MID49 4xMyc Hisx6 was a gift from David Chan (Addgene plasmid # 44596 ; http://n2t.net/addgene:44596 ; RRID:Addgene_44596)

  • For your References section:

    Fis1, Mff, MiD49 and MiD51 mediate Drp1 recruitment in mitochondrial fission. Loson OC, Song Z, Chen H, Chan DC. Mol Biol Cell. 2013 Jan 2. 10.1091/mbc.E12-10-0721 PubMed 23283981
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