pDONR207-Citrine-PTS1
(Plasmid
#44588)
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PurposeGateway entry vector for Citrine fluorescent protein with a peroxisome localization signal
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 44588 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR207
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 3373
- Total vector size (bp) 4102
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCitrine-PTS1
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SpeciesSynthetic
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Insert Size (bp)729
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Tag
/ Fusion Protein
- PTS1 (peroxisomal targeting signal type 1)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer TCGCGTTAACGCTAGCATGGATCTC
- 3′ sequencing primer GTAACATCAGAGATTTTGAGACAC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR207-Citrine-PTS1 was a gift from Yutaka Kodama (Addgene plasmid # 44588 ; http://n2t.net/addgene:44588 ; RRID:Addgene_44588) -
For your References section:
Cold-induced organelle relocation in the liverwort Marchantia polymorpha L. Ogasawara Y, Ishizaki K, Kohchi T, Kodama Y. Plant Cell Environ. 2013 Feb 20. doi: 10.1111/pce.12085. 10.1111/pce.12085 PubMed 23421791