K18iresCreER-T2
(Plasmid #44580)

• Depositing Lab: Robert Oshima
• Publication: Wen et al Genesis. 2003 Feb;35(2):100-6.

Backbone

• Vector backbone: pGEM-1
• Total vector size (bp): 15682
• Vector type: Mouse Targeting

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: keratin 18 gene
• Species: H. sapiens (human)
• Entrez Gene: KRT18 (a.k.a. CK-18, CYK18, K18)
• Tag / Fusion Protein:
  • IRES_CreER-T2

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NA (unknown if destroyed)
• 3′ cloning site: NA (unknown if destroyed)
• 5′ sequencing primer: Sp6
• 3′ sequencing primer: T7

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• A portion of this plasmid was derived from a plasmid made by: CreER was provided by Dr. Pierre Chambon.
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Sanford-Burnham Medical Research Institute Ancillary Agreement
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Cre recombinase-mutant estrogen receptor fusion (CreERT2) in K18 gene-ires vector for tamoxifen-dependent Cre activity confined to epithelial tissues. This is the full length K18 gene with an IRES inserted downstream of sixth exon TGA and before the polyA signal.

Please note that the partial plasmid sequence available for this plasmid is missing the human ERT2 sequence. This has been indicated in the partial sequence by a string of 10 N's at bp#7941-7950 immediately after the Cre sequence. See Addgene's sequencing result with the Cre-F primer for more information about this region.

Human K18 gene from pGC1853 (Addgene #44619).