Skip to main content
Addgene

pLPC-Flag-TRF2(delta)iDDR
(Plasmid #44577)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44577 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pLPC
  • Total vector size (bp) 6943
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TRF2ΔiDDR
  • Alt name
    TRF2deltaiDDR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1428
  • Mutation
    iDDR domain (aa 407-431) has been deleted
  • GenBank ID
    NM_005652.3 NP_005643.1
  • Entrez Gene
    TERF2 (a.k.a. TRBF2, TRF2)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pLPC-5
  • 3′ sequencing primer pLPC-3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

TRF2 construct with the iDDR region removed (aa 407-431 when numbered according to GenBank accession number NP_005643.1). Deleted protein sequence is: PKGKWNSSNGVEEKETWVEEDELFQ

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLPC-Flag-TRF2(delta)iDDR was a gift from Eros Lazzerini Denchi (Addgene plasmid # 44577 ; http://n2t.net/addgene:44577 ; RRID:Addgene_44577)

  • For your References section:

    A two-step mechanism for TRF2-mediated chromosome-end protection. Okamoto K, Bartocci C, Ouzounov I, Diedrich JK, Yates Iii JR, Denchi EL. Nature. 2013 Feb 6. doi: 10.1038/nature11873. 10.1038/nature11873 PubMed 23389450
Related items:
Commonly requested with: