pBabe-Myc-TRF1iDDR
(Plasmid
#44576)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 44576 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBabe
- Total vector size (bp) 6528
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pBabe 5'
- 3′ sequencing primer pBabe 3' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
TRF chimera with iDDR region of TRF2 (aa 407-431 compared to GenBank accession number NP_005643.1 - PKGKWNSSNGVEEKETWVEEDELFQ) replacing aa 346-364 of TRF1 (NP_059523.2).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBabe-Myc-TRF1iDDR was a gift from Eros Lazzerini Denchi (Addgene plasmid # 44576 ; http://n2t.net/addgene:44576 ; RRID:Addgene_44576) -
For your References section:
A two-step mechanism for TRF2-mediated chromosome-end protection. Okamoto K, Bartocci C, Ouzounov I, Diedrich JK, Yates Iii JR, Denchi EL. Nature. 2013 Feb 6. doi: 10.1038/nature11873. 10.1038/nature11873 PubMed 23389450