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Addgene

pLPC-Myc-TRFcT
(Plasmid #44573)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44573 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLPC
  • Total vector size (bp) 7051
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    chimeric TRF
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1515
  • Mutation
    TRF2 where TRFH domain (aa42-241) has been replaced by that of TRF1 (aa58-262); A434 deleted
  • GenBank ID
    NP_005643.1 for TRF2 NP_059523.2 for TRF1
  • Entrez Gene
    TERF2 (a.k.a. TRBF2, TRF2)
  • Entrez Gene
    TERF1 (a.k.a. PIN2, TRBF1, TRF, TRF1, hTRF1-AS, t-TRF1)
  • Tag / Fusion Protein
    • Myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pLPC-5
  • 3′ sequencing primer pLPC-3
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

TRF2 construct in which the TRFH domain of TRF2 (aa42-241 when compared to NP_005643.1) was replaced with TRFH domain of TRF1 (aa58-262 when compared to NP_059523.2).

According to the depositing laboratory, the deletion of A434 does not affect TRF2 function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLPC-Myc-TRFcT was a gift from Eros Lazzerini Denchi (Addgene plasmid # 44573 ; http://n2t.net/addgene:44573 ; RRID:Addgene_44573)

  • For your References section:

    A two-step mechanism for TRF2-mediated chromosome-end protection. Okamoto K, Bartocci C, Ouzounov I, Diedrich JK, Yates Iii JR, Denchi EL. Nature. 2013 Feb 6. doi: 10.1038/nature11873. 10.1038/nature11873 PubMed 23389450
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