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Addgene

MAVS-Mito
(Plasmid #44556)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44556 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMSCV2.2 IRES GFP
  • Backbone size w/o insert (bp) 6600
  • Total vector size (bp) 8200
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MAVS-Fis1 chimeric protein
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1600
  • Mutation
    contains aa1-500 of MAVS and aa127-152 of Fis1
  • GenBank ID
    BC044952 and AAH44952.1 for MAVS. NM_016068 and NP_057152.2 for Fis1.
  • Entrez Gene
    MAVS (a.k.a. CARDIF, IPS-1, IPS1, VISA)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pMSCV forward
  • 3′ sequencing primer MAVS scn
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    ZJ Chen provided HA-MAVS in pEF
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MAVS-Mito was a gift from Jonathan Kagan (Addgene plasmid # 44556 ; http://n2t.net/addgene:44556 ; RRID:Addgene_44556)

  • For your References section:

    Peroxisomes are signaling platforms for antiviral innate immunity. Dixit E, Boulant S, Zhang Y, Lee AS, Odendall C, Shum B, Hacohen N, Chen ZJ, Whelan SP, Fransen M, Nibert ML, Superti-Furga G, Kagan JC. Cell. 2010 May 14;141(4):668-81. Epub 2010 May 6. 10.1016/j.cell.2010.04.018 PubMed 20451243
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