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Addgene

pCM350
(Plasmid #44549)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44549 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS416
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HA-htz1
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    deleted amino acids 1-22
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer M13_Rev
  • 3′ sequencing primer M13_F20
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCM350 was a gift from Michael Grunstein (Addgene plasmid # 44549 ; http://n2t.net/addgene:44549 ; RRID:Addgene_44549)

  • For your References section:

    Acetylation of H2AZ Lys 14 is associated with genome-wide gene activity in yeast. Millar CB, Xu F, Zhang K, Grunstein M. Genes Dev. 2006 Mar 15;20(6):711-22. 10.1101/gad.1395506 PubMed 16543223
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