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Addgene

pFX21
(Plasmid #44533)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44533 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-24a(+)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SIR2-6xHis
  • Species
    S. cerevisiae (budding yeast)
  • Tag / Fusion Protein
    • His6 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe I (unknown if destroyed)
  • 3′ cloning site Xho I (unknown if destroyed)
  • 5′ sequencing primer T7 promoter primer
  • 3′ sequencing primer T7 terminator primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFX21 was a gift from Michael Grunstein (Addgene plasmid # 44533 ; http://n2t.net/addgene:44533 ; RRID:Addgene_44533)

  • For your References section:

    Sir2 deacetylates histone H3 lysine 56 to regulate telomeric heterochromatin structure in yeast. Xu F, Zhang Q, Zhang K, Xie W, Grunstein M. Mol Cell. 2007 Sep 21;27(6):890-900. 10.1016/j.molcel.2007.07.021 PubMed 17889663
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