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Addgene

pFX05
(Plasmid #44531)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44531 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRM200
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HHT2
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    K56Q

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH I (unknown if destroyed)
  • 3′ cloning site Sal I (unknown if destroyed)
  • 5′ sequencing primer CGCGTTGCTTCTTGTGACCGCAGTTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFX05 was a gift from Michael Grunstein (Addgene plasmid # 44531 ; http://n2t.net/addgene:44531 ; RRID:Addgene_44531)

  • For your References section:

    Sir2 deacetylates histone H3 lysine 56 to regulate telomeric heterochromatin structure in yeast. Xu F, Zhang Q, Zhang K, Xie W, Grunstein M. Mol Cell. 2007 Sep 21;27(6):890-900. 10.1016/j.molcel.2007.07.021 PubMed 17889663
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