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Addgene

pRM415
(Plasmid #44526)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44526 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLJ999T
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HHT2
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    hht2 deletion 4-15

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH I (unknown if destroyed)
  • 3′ cloning site Sal I (unknown if destroyed)
  • 5′ sequencing primer CGCGTTGCTTCTTGTGACCGCAGTTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRM415 was a gift from Michael Grunstein (Addgene plasmid # 44526 ; http://n2t.net/addgene:44526 ; RRID:Addgene_44526)

  • For your References section:

    Histone H3 N-terminal mutations allow hyperactivation of the yeast GAL1 gene in vivo. Mann RK, Grunstein M. EMBO J. 1992 Sep;11(9):3297-306. PubMed 1505519
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