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Addgene

p296.2
(Plasmid #44519)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44519 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX2T
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Histone 2A1 aa 1-35
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    aa 1-35
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer pGEX_3 primer
  • 3′ sequencing primer M13-pUC-Rev-primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p296.2 was a gift from Michael Grunstein (Addgene plasmid # 44519 ; http://n2t.net/addgene:44519 ; RRID:Addgene_44519)

  • For your References section:

    Histone H3 and H4 N-termini interact with SIR3 and SIR4 proteins: a molecular model for the formation of heterochromatin in yeast. Hecht A, Laroche T, Strahl-Bolsinger S, Gasser SM, Grunstein M. Cell. 1995 Feb 24;80(4):583-92. 10.1016/0092-8674(95)90512-X PubMed 7867066
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