pMMB66EH-aphB
(Plasmid
#44496)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 44496 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMMB66EH
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Backbone manufacturerAATC
- Backbone size w/o insert (bp) 8807
- Total vector size (bp) 9683
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Modifications to backbonenone
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsWill also grow at 30deg in Vibrio cholerae.
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameAphB
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Alt nameVC1049 (N16961)
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SpeciesVibrio cholerae
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Insert Size (bp)876
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Entrez GeneVC1049 (a.k.a. VC1049)
- Promoter tac
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer GGA ATT GTG AGC GGA TAA CA
- 3′ sequencing primer GAT TTA ATC TGT ATC AGG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMMB66EH-aphB was a gift from Victor DiRita (Addgene plasmid # 44496 ; http://n2t.net/addgene:44496 ; RRID:Addgene_44496) -
For your References section:
Small-molecule inhibitors of toxT expression in Vibrio cholerae. Anthouard R, DiRita VJ. MBio. 2013 Aug 6;4(4). pii: e00403-13. doi: 10.1128/mBio.00403-13. 10.1128/mBio.00403-13 PubMed 23919997
