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pCFJ1319
(Plasmid #44495)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44495 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDESTR4-R3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2445
  • Vector type
    Gateway expression clone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    EPI400
  • Growth instructions
    The plasmid was transformed into EPI400 cells from Epicentre and requires induction with their "copy cutter solution" for DNA isolation.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Minimal Mos1 [256x LacO, unc-119, PuroR, peel-1]
  • Species
    D. melanogaster (fly), C. elegans (nematode); Bacterial
  • Insert Size (bp)
    12458

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid may contain truncated lacO repeats. The depositing lab is re-transforming the plasmid and will deposit new verified strain.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCFJ1319 was a gift from Erik Jorgensen (Addgene plasmid # 44495)

  • For your References section:

    Random and targeted transgene insertion in Caenorhabditis elegans using a modified Mos1 transposon. Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella M, Pozniakovsky A, Moerman DG, Jorgensen EM. Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 2014 Mar 16. 10.1038/nmeth.2889 PubMed 24820376
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