pCFJ1324
(Plasmid
#44494)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 44494 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDESTR4-R3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2445
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Vector typeGateway expression clone
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)EPI400
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Growth instructionsThe plasmid was transformed into EPI400 cells from Epicentre and requires induction with their "copy cutter solution" for DNA isolation.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMinimal Mos1 [256x LacO, unc-119, NeoR, peel-1]
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SpeciesD. melanogaster (fly), C. elegans (nematode); Bacterial
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Insert Size (bp)12458
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13rev
- 3′ sequencing primer M13-fwd20 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid may contain truncated lacO repeats. The depositing lab is re-transforming the plasmid and will deposit new verified strain.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCFJ1324 was a gift from Erik Jorgensen (Addgene plasmid # 44494) -
For your References section:
Random and targeted transgene insertion in Caenorhabditis elegans using a modified Mos1 transposon. Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella M, Pozniakovsky A, Moerman DG, Jorgensen EM. Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 2014 Mar 16. 10.1038/nmeth.2889 PubMed 24820376