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Addgene

pCFJ914
(Plasmid #44489)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44489 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDESTR4-R3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2445
  • Vector type
    Gateway expression clone
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Minimal Mos1 [Peft-3 GFP H2B tbb-2 UTR, NeoR]
  • Species
    D. melanogaster (fly), C. elegans (nematode); Bacterial
  • Insert Size (bp)
    2278

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCFJ914 was a gift from Erik Jorgensen (Addgene plasmid # 44489 ; http://n2t.net/addgene:44489 ; RRID:Addgene_44489)

  • For your References section:

    Random and targeted transgene insertion in Caenorhabditis elegans using a modified Mos1 transposon. Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella M, Pozniakovsky A, Moerman DG, Jorgensen EM. Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 2014 Mar 16. 10.1038/nmeth.2889 PubMed 24820376
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