pGreenII 0579-1 (35S::LUC)
(Plasmid #44468)

• Depositing Lab: Roger Hellens
• Publication: Plasmids to assay for nuclease cleavage in planta (unpublished)

Backbone

• Vector backbone: pGreenII 0000
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 5544
• Vector type: Plant Transformation

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: A luciferase gene
• Alt name: LUC
• Alt name: Firefly-derived Luciferase
• Species: Synthetic
• Promoter: 35S Promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: HpaI (destroyed during cloning)
• 5′ sequencing primer: RAJ-198 (CTTAGTTTACCCGCCAATATATCCTGTCA)
• 3′ sequencing primer: RAJ-199 (AGATCTTGGCAGGATATATTGTGGTGTAAC)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This plasmid was derived from pGreenII 0800, which was obtained from Roger P. Hellens from the New Zealand Institute for Plant Breeding Research Limited
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Reference: Johnson RA, Hellens RP, Love DR (2011) A transient assay for recombination demonstrates that Arabidopsis SNM1 and XRCC3 enhance non-homologous recombination. Genetics and Molecular Research 10 (3):2104-2132. doi:10.4238/vol10-3gmr1347