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Addgene

pcDNA3 Connexin43-APEX
(Plasmid #44439)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44439 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Total vector size (bp) 7340
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Connexin43 fused to pea APEX
  • Species
    Pea
  • Insert Size (bp)
    1974
  • Mutation
    K14D, W41F, E112K mutations on APEX
  • Promoter CMV
  • Tag / Fusion Protein
    • myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGHrev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 Connexin43-APEX was a gift from Alice Ting (Addgene plasmid # 44439 ; http://n2t.net/addgene:44439 ; RRID:Addgene_44439)

  • For your References section:

    Engineered ascorbate peroxidase as a genetically encoded reporter for electron microscopy. Martell JD, Deerinck TJ, Sancak Y, Poulos TL, Mootha VK, Sosinsky GE, Ellisman MH, Ting AY. Nat Biotechnol. 2012 Oct 21. doi: 10.1038/nbt.2375. 10.1038/nbt.2375 PubMed 23086203
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