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Neurod1-Atoh7HA3
(Plasmid #44438)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44438 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBlueScript II KS(+)
  • Backbone manufacturer
    Stratagene
  • Vector type
    Mammalian Expression, Mouse Targeting, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Atoh7-3xHA knock-in
  • Alt name
    atonal homolog 7 (Drosophila) with 3xHA tag
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Atoh7 (a.k.a. Math5, bHLHa13)
  • Tags / Fusion Proteins
    • Neurod1 left arm (N terminal on insert)
    • loxC2-Reverse Pgk-neo-TpA-loxC2 (N terminal on insert)
    • Neurod1 right arm (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site N/A (unknown if destroyed)
  • 5′ sequencing primer T7 Fwd
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Neurod1-Atoh7(Math5)HA3 knock-in targeting construct, for replacing Neurod1 with Atoh7HA3. Note: the full sequence for this plasmid is approximated and not fully verified.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Neurod1-Atoh7HA3 was a gift from William Klein (Addgene plasmid # 44438 ; http://n2t.net/addgene:44438 ; RRID:Addgene_44438)

  • For your References section:

    Reprogramming amacrine and photoreceptor progenitors into retinal ganglion cells by replacing Neurod1 with Atoh7. Mao CA, Cho JH, Wang J, Gao Z, Pan P, Tsai WW, Frishman LJ, Klein WH. Development. 2013 Feb;140(3):541-51. doi: 10.1242/dev.085886. 10.1242/dev.085886 PubMed 23293286
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