pcDNA3.1 CARMA1
(Plasmid #44431)

• Depositing Lab: Xin Lin
• Publication: Xin Lin Lab Plasmids (unpublished)

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 8800
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CARMA1
• Alt name: caspase recruitment domain family, member 11
• Species: H. sapiens (human)
• Insert Size (bp): 3400
• Mutation: See depositor comments below.
• Entrez Gene: CARD11 (a.k.a. BENTA, BIMP3, CARMA1, IMD11, IMD11A, PPBL)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-Rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene NGS results found a S286C variant compared to NCBI reference AAI11720.2.